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The Ralph Beckett-trained Cubanita (8-1) collared Sajjhaa in the dying strides to snatch the Betfred EBF Gillies Fillies' Stakes at Doncaster.
Godolphin's highly-rated Sajjhaa looked to have the race won when Mickael Barzalona kicked on approaching two out.
But her stride shortened as Cubanita's stamina kicked-in entering the distance, and Jim Crowley's mount was half a length to the good at the line.
Crowley said: "It's been stop-start with her this season so hopefully she'll stay in training next year, fingers crossed.
"I got a good trip round. They went a good gallop and everything went well in the end.
"I think she'll get a mile and a half. The last furlong was her best furlong."
Godolphin clinched the owners' championship with a double through Mar Mar and Muharrib, both trained by Saeed bin Suroor.
Mar Mar (7-2) showed a neat turn of foot two furlongs out to easily win the Betfred Cock O'The North EBF Maiden Stakes by four lengths under William Buick.
"She's a very honest filly, but she was a bit unsettled and keen last time, so we thought visors would help. She'll be a nice seven-furlong filly for next year," said Godolphin's representative Diana Cooper.
Muharrib (5-1) led over a furlong out under Barzalona to defy top weight in the Betfred "The Home Of Goals Galore" Nursery.
Cooper said: "He's a very bonny horse, who has thrived all season. He'll have a nice holiday now and he's one to look forward to. He's very honest and game and gives his all every time."
Stuart Williams' decision to bypass Ascot's Champions Sprint paid off when Eton Rifles (7-1) took the "Betfred Bonus King Bingo" Wentworth Stakes.
The Newmarket trainer felt the seven-year-old was unlucky in this Listed contest 12 months ago.
Eton Rifles made amends with a length verdict over Ultransonic in the hands of William Carson.
"He had a nice run through the race, but he was a close third to Mince and Soul last time and if you had put them in this race with that weight they would have been favourites," said Williams.
"We saved him for this race. We could have gone to Ascot for the Group Two, but we thought we'd come here and hopefully win.
"He was very unlucky in this race last year just after we bought him. He's a superstar for a yard my size.
"He didn't have much racing in his younger years and he's very genuine. We might come back here for the Cammidge Trophy in the spring."
The Dandy Nicholls-trained Desert Creek (6-1) gave Shirley Teasdale her 19th winner in division one of the Betfred "Bigger And Better" Goals Galore Apprentice Handicap but the rider was banned for two days (November 24 and 26) for careless riding.
Chookie Royale (13-2 favourite) took division two for trainer and former jockey Keith Dalgleish in the hands of Jason Hart.
Matthew Hopkins, rider of the third Fame Again was banned for a total of six days (November 24 and 26-30) for careless riding and using his whip above the permitted level.
Franny Norton, successful in the November Handicap, completed a double in the final race of the Flat turf season, taking the Betfred "The Bonus King" Handicap on Tartiflette (10-1) for trainer Ed McMahon.