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Seventh seed Pauline Parmentier was knocked out by qualifier Tereza Mrdeza as the first round of the Copa Colsanitas continued in Bogota.
The Frenchwoman was beaten in straight sets by her Croatian opponent, who claimed a 7-6(5) 6-4 win.
That aside the seeds fared well.
Third seed Lourdes Dominguez defeated Colombian Catalina Castano 3-6 6-2 6-1, while a second home player, Yuliana Lizarazo, went down 6-2 1-6 6-3 to Lara Arruabarrena-Vecino.
Italian pair Francesca Schiavone and Flavia Pennetta, seeded fourth and fifth, both won in three sets seeing off Sharon Fichman and Alexandra Panova respectively.
Eighth seed Timeas Babos of Hungary defeated Beatriz Garcia Vidagany 6-4 1-6 7-6(4).
Also into the second round are Brazil's Teliana Pereira, Romanian Alexandra Cadantu, Spain's Maria-Teresa Torro-Flor and Italian Karin Knapp.