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Nathaniel will miss the Prix de l'Arc de Triomphe after suffering a setback, confirmed James Wigan, racing manager to owner Lady Rothschild.
The four-year-old colt, trained by John Gosden, is out of Europe's premier middle-distance race after returning unsatisfactory blood tests when he was found to have had a temperature.
Wigan said: "He had a temperature this morning, they took some blood tests and his blood wasn't right.
"Considering how close we are to the race, we cannot consider running him."
Nathaniel's defection is another big blow to this year's Arc after last season's winner Danedream was withdrawn on Tuesday morning when her stables at Cologne racecourse were placed into quarantine on Monday.
Snow Fairy was also taken out of Sunday's Longchamp race at the weekend.
Fellow part-owner Newsells Park Stud were also left cursing their bad luck after Nathaniel's Arc dream was ended.
General manager Julian Dollar tweeted: "Nathaniel a non runner in Arc due to a high temp and poor bloods. I'm using every profanity I know right now, plus a few I've just invented!"